Model Protein Standards

• Superimposed NMR spectra of 2H13C15N-labelled ubiquitin (blue) and 13C15N-labelled ubiquitin (red)
• Superimposed NMR spectra of 2H13C15N-labelled ubiquitin (blue) and 2H15N-labelled ubiquitin (red)
• Superimposed NMR spectra of 2H13C15N-labelled ubiquitin (blue) and 15N-labelled ubiquitin (red)
• Superimposed NMR spectra of 13C15N-labelled ubiquitin (blue) and 2H15N-labelled ubiquitin (red)
• Superimposed NMR spectra of 13C15N-labelled ubiquitin (blue) and 15N-labelled ubiquitin (red)
• Superimposed NMR spectra of 2H15N-labelled ubiquitin (blue) and 15N-labelled ubiquitin (red)
• Superimposed NMR spectra of 15N-labelled ubiquitin (blue), 2H15N-labelled ubiquitin (red), 13C15N-labelled ubiquitin (magenta) and 2H13C15N-labelled ubiquitin (yellow)

Use cases of the Silantes ubiquitin in scientific publications:

• Association between CSF alpha-synuclein seeding activity and genetic status in Parkinson’s disease and dementia with Lewy bodies. (n.d.). http://springermedizin.de . https://www.springermedizin.de/association-between-csf-alpha-synuclein-seeding-activity-and-gen/25667868
• Yuan, X., Simpson, P., Mckeown, C., Kondo, H., Uchiyama, K., Wallis, R., Dreveny, I., Keetch, C., Zhang, X., Robinson, C., Freemont, P., & Matthews, S. (2004). Structure, dynamics and interactions of p47, a major adaptor of the AAA ATPase, p97. The EMBO Journal, 23(7), 1463–1473. https://doi.org/10.1038/sj.emboj.7600152
• Haller, J. D., Bodor, A., & Luy, B. (2022). Pure shift amide detection in conventional and TROSY-type experiments of 13C,15N-labeled proteins. Journal of Biomolecular NMR, 76(5–6), 213–221. https://doi.org/10.1007/s10858-022-00406-z
• Toft, A., Sjödin, S., Simonsen, A. H., Ejlerskov, P., Roos, P., Musaeus, C. S., Henriksen, E. E., Nielsen, T. T., Brinkmalm, A., Blennow, K., Zetterberg, H., & Nielsen, J. E. (2023). Endo‐lysosomal protein concentrations in CSF from patients with frontotemporal dementia caused by CHMP2B mutation. Alzheimer S & Dementia Diagnosis Assessment & Disease Monitoring, 15(1). https://doi.org/10.1002/dad2.12402
• Sjödin, S., Hansson, O., Öhrfelt, A., Brinkmalm, G., Zetterberg, H., Brinkmalm, A., & Blennow, K. (2017). Mass spectrometric analysis of cerebrospinal fluid ubiquitin in Alzheimer’s disease and parkinsonian disorders. PROTEOMICS – CLINICAL APPLICATIONS, 11(11–12). https://doi.org/10.1002/prca.201700100
• Lowe, A. J., Sjödin, S., Rodrigues, F. B., Byrne, L. M., Blennow, K., Tortelli, R., Zetterberg, H., & Wild, E. J. (2020). Cerebrospinal fluid endo-lysosomal proteins as potential biomarkers for Huntington’s disease. PLoS ONE, 15(8), e0233820. https://doi.org/10.1371/journal.pone.0233820