Lys(6)-SILAC-Mouse Diet (Worldwide only provided by Silantes GmbH)
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A New Silantes Proteomics Product
SILAC-mouse is a new in vivo proteomics approach (Ong et Mann 2006) permitting quantitative determination of protein patterns of mouse organs. The concept is similar to that of the well established SILAC-approach (Ong et al. 2002) aiming at the quantification of proteins in cell cultures. The workflow scheme of SILAC-mouse is shown below.
In order to determine the protein status of mouse (A) with respect to mouse (B), tissues of mice (A) and (B), both of which were fed a diet containing only unlabelled (12C6) lysine, are each mixed with tissue of mouse (R) which was fed a diet in which 12C6 lysine has been replaced by 13C6-lysine.
Silantes, in cooperation with the group of Prof. Matthias Mann, Max-Planck-Institute of Biochemistry, has developed a kit for performing the SILAC-mouse approach (Krüger et al.2008).
The Lys(6)-SILAC-Mouse Diet Kit consists of two feeds:
A feed (from Harlan) which is composed of a conventional amino acid-based diet containing 12C6-lysine (light feed). This diet is used for feeding mice (A) and (B);
The same feed as above but 12C6-lysine is replaced by 13C6-lysine (heavy feed). This diet is used for feeding the reference mouse (R).
Alternatively, the Lys(8)-SILAC-Mouse Diet Kit is available in which 13C6-lysine is replaced by 13C6,15N2-lysine yielding an 8 Da molecular weight shift of the proteolytically cleaved peptides.
Krueger M., Moser M., Ussar S., Thievessen I., Luber Ch.A, Forner F., Schmidt S., Zanivan S., Faessler R. and Mann M.(2008). SILAC Mouse for Quantitative Proteomics Uncovers Kindlin-3 as an Essential Factor for Red Blood Cell Function, Cell 134, 353–364.
Ong, S.E., and Mann, M. (2006). A practical recipe for stable isotope labelling by amino acids in cell culture (SILAC). Nat. Protoc. 1, 2650–2660.
Ong, S.E., Blagoev, B., Kratchmarova, I., Kristensen, D.B., Steen, H., Pandey A., and Mann, M. (2002). Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics, Mol.Cell. Proteomics 1, 376–386.
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