Stable Isotope labeled Nucleosidetriphosphates

Structural studies on large DNA and RNA molecules by nuclear magnetic resonance (NMR) require labeling of these molecules with stable isotopes. Silantes offers a wide range of stable isotope labeled ribo- and deoxyribo-nucleosidetriphosphates (rNTP, dNTP) for the synthesis of oligonucleotides.
 

Silantes Technology

Silantes dNTPs and rNTPs are made from DNA and RNA purified from bacteria. The bacterial strain used is a chemolithoautotrophic organism which grows on isotopically labeled H2, O2 and CO2. The extracted RNA or DNA is enzymatically hydrolysed. The 5'-monophosphates are enzymatically phosphorylated into 5'-dNTPs and 5'-rNTP and purified by ion and reversed phase HPLC.
 

Added Value

Our new technology for in vivo enrichment of stable isotopes enables us to offer the ribo- and deoxyribo-nucleosidetriphosphates at very competitive prices.
 

Isotope Enrichment

Silantes NTPs can be supplied in any combination of 2H, 13C and 15N.
The isotopic purity for all isotopes is >98%.
 

Quality

The quality of dNTPs and rNTPs is tested with respect to chemical purity (>95%) (Fig. 1) and biological competence analysed by PCR. NTPs are available in solution (C = 100 mM in 5 mM TrisHCL, pH 7.5).
 

Conclusion

Silantes offers a full range of stable isotope labeled ribo- and deoxyribo-nucleoside-triphosphates:

·  with full biological competence
·  of >98% isotopic purity
·  of >95% chemical purity
·  at very competitive prices

NTPs

"We are very happy with the 13C, 15N-labeled RNA nucleotides that we have obtained from Silantes. We consistently receive samples of high purity that afford high yields when used in in vitro transcription reactions in our lab. It is also significant that we have been able to receive relatively large amounts of labeled ribo-NTPs with minimal delays from the time that orders are placed to the time the samples arrive in our lab (typically less than 2-3 weeks)."
Michael F. Summers, Howard Hughes Medical Institute, Chevy Chase, USA

 

 

"The 13C, 15N labeled NTPs provided by Silantes were of high quality and worked very well in our hands. I can strongly recommend to use the products by Silantes."
Prof. Harald Schwalbe, Massachusetts Institute of Technology, Department of Chemistry, Cambridge, MA, USA

 

 

"We are really pleased by the quality of the SILANTES 13C, 15N-labeled rNTPs. At a very resonable price, they reproducibely give yields comparable to optimized in vitro transcription reactions using commercially available unlabeled rNTPs. No further purification needed! And equally important, SILANTES typically delivered with minimal delays even when we ordered larger quantities."
Mirko Hennig & James R. Williamson, The Scripps Research Institute, La Jolla, CA

 

Product-N°: Description labeled
(> 98%)
Quantity  
120103601 dATP D 2H 10 mg  
120103602 dCTP D 2H 10 mg  
120103603 dGTP D 2H 10 mg  
120103604 dTTP D 2H 10 mg  
120106100 dNTP - Set D (GATC) 2H 4 x 10 mg  
         
120103801 dATP D 2H 50 mg  
120103802 dCTP D 2H 50 mg  
120103803 dGTP D 2H 50 mg  
120103804 dTTP D 2H 50 mg  
120106200 dNTP - Set D (GATC) 2H 4 x 50 mg  
         
120303601 dATP N 15N 10 mg  
120303602 dCTP N 15N 10 mg  
120303603 dGTP N 15N 10 mg  
120303604 dTTP N 15N 10 mg  
120306100 dNTP - Set N (GATC) 15N 4 x 10 mg  
         
120303801 dATP N 15N 50 mg  
120303802 dCTP N 15N 50 mg  
120303803 dGTP N 15N 50 mg  
120303804 dTTP N 15N 50 mg  
120306200 dNTP - Set N (GATC) 15N 4 x 50 mg  
         
120603601 dATP CN 13C, 15N 10 mg  
120603602 dCTP CN 13C, 15N 10 mg  
120603603 dGTP CN 13C, 15N 10 mg  
120603604 dTTP CN 13C, 15N 10 mg  
120606100 dNTP - Set CN (GATC) 13C, 15N 4 x 10 mg  
         
120603801 dATP CN 13C, 15N 50 mg  
120603802 dCTP CN 13C, 15N 50 mg  
120603803 dGTP CN 13C, 15N 50 mg  
120603804 dTTP CN 13C, 15N 50 mg  
120606200 dNTP - Set CN (GATC) 13C, 15N 4 x 50 mg  
         
 
Product-N°: Description labeled
(> 98%)
Quantity  
122103601 dAMP D 2H 10 mg  
122103602 dCMP D 2H 10 mg  
122103603 dGMP D 2H 10 mg  
122103604 dTMP D 2H 10 mg  
         
122103801 dAMP D 2H 50 mg  
122103802 dCMP D 2H 50 mg  
122103803 dGMP D 2H 50 mg  
122103804 dTMP D 2H 50 mg  
         
122303601 dAMP N 15N 10 mg  
122303602 dCMP N 15N 10 mg  
122303603 dGMP N 15N 10 mg  
122303604 dTMP N 15N 10 mg  
         
122303801 dAMP N 15N 50 mg  
122303802 dCMP N 15N 50 mg  
122303803 dGMP N 15N 50 mg  
122303804 dTMP N 15N 50 mg  
         
122603601 dAMP CN 13C, 15N 10 mg  
122603602 dCMP CN 13C, 15N 10 mg  
122603603 dGMP CN 13C, 15N 10 mg  
122603604 dTMP CN 13C, 15N 10 mg  
         
122603801 dAMP CN 13C, 15N 50 mg  
122603802 dCMP CN 13C, 15N 50 mg  
122603803 dGMP CN 13C, 15N 50 mg  
122603804 dTMP CN 13C, 15N 50 mg  
         
 
Product-N°: Description labeled
(> 98%)
Quantity  
124303601 dA N 15N 10 mg  
124303602 dC N 15N 10 mg  
124303603 dG N 15N 10 mg  
124303604 dT N 15N 10 mg  
         
124303801 dA N 15N 50 mg  
124303802 dC N 15N 50 mg  
124303803 dG N 15N 50 mg  
124303804 dT N 15N 50 mg  
         
124603601 dA CN 13C, 15N 10 mg  
124603602 dC CN 13C, 15N 10 mg  
124603603 dG CN 13C, 15N 10 mg  
124603604 dT CN 13C, 15N 10 mg  
         
124603801 dA CN 13C, 15N 50 mg  
124603802 dC CN 13C, 15N 50 mg  
124603803 dG CN 13C, 15N 50 mg  
124603804 dT CN 13C, 15N 50 mg  
         
The following dNTPs will be available soon:    
         
120203601 dATP C 13C 10 mg  
120203602 dCTP C 13C 10 mg  
120203603 dGTP C 13C 10 mg  
120203604 dTTP C 13C 10 mg  
120206100 dNTPs - Set C (GATC) 13C 4 x 10 mg  
         
120203801 dATP C 13C 50 mg  
120203802 dCTP C 13C 50 mg  
120203803 dGTP C 13C 50 mg  
120203804 dTTP C 13C 50 mg  
120206200 dNTPs - Set C (GATC) 13C 4 x 50 mg  
         
 
Product-N°: Description labeled
(> 98%)
Quantity  
121103601 rATP D 2H 10 mg  
121103602 rCTP D 2H 10 mg  
121103603 rGTP D 2H 10 mg  
121103604 rUTP D 2H 10 mg  
121106100 rNTP - Set D (GAUC) 2H 4 x 10 mg  
         
121103801 rATP D 2H 50 mg  
121103802 rCTP D 2H 50 mg  
121103803 rGTP D 2H 50 mg  
121103804 rUTP D 2H 50 mg  
121106200 rNTP - Set D (GAUC) 2H 4 x 50 mg  
         
121303601 rATP N 15N 10 mg  
121303602 rCTP N 15N 10 mg  
121303603 rGTP N 15N 10 mg  
121303604 rUTP N 15N 10 mg  
121306100 rNTPs - Set N (GAUC) 15N 4 x 10 mg  
         
121303801 rATP N 15N 50 mg  
121303802 rCTP N 15N 50 mg  
121303803 rGTP N 15N 50 mg  
121303804 rUTP N 15N 50 mg  
121306200 rNTPs - Set N (GAUC) 15N 4 x 50 mg  
         
121603601 rATP CN 13C, 15N 10 mg  
121603602 rCTP CN 13C, 15N 10 mg  
121603603 rGTP CN 13C, 15N 10 mg  
121603604 rUTP CN 13C, 15N 10 mg  
121606100 rNTPs - Set CN (GAUC) 13C, 15N 4 x 10 mg  
         
121603801 rATP CN 13C, 15N 50 mg  
121603802 rCTP CN 13C, 15N 50 mg  
121603803 rGTP CN 13C, 15N 50 mg  
121603804 rUTP CN 13C, 15N 50 mg  
121606200 rNTPs - Set CN (GAUC) 13C, 15N 4 x 50 mg  
         
         
Product-N°: Description labeled
(> 98%)
Quantity  
123103601 rAMP D 2H 10 mg  
123103602 rCMP D 2H 10 mg  
123103603 rGMP D 2H 10 mg  
123103604 rUMP D 2H 10 mg  
         
123103801 rAMP D 2H 50 mg  
123103802 rCMP D 2H 50 mg  
123103803 rGMP D 2H 50 mg  
123103804 rUMP D 2H 50 mg  
         
123303601 rAMP N 15N 10 mg  
123303602 rCMP N 15N 10 mg  
123303603 rGMP N 15N 10 mg  
123303604 rUMP N 15N 10 mg  
         
123303801 rAMP N 15N 50 mg  
123303802 rCMP N 15N 50 mg  
123303803 rGMP N 15N 50 mg  
123303804 rUMP N 15N 50 mg  
         
123603601 rAMP CN 13C, 15N 10 mg  
123603602 rCMP CN 13C, 15N 10 mg  
123603603 rGMP CN 13C, 15N 10 mg  
123603604 rUMP CN 13C, 15N 10 mg  
         
123603801 rAMP CN 13C, 15N 50 mg  
123603802 rCMP CN 13C, 15N 50 mg  
123603803 rGMP CN 13C, 15N 50 mg  
123603804 rUMP CN 13C, 15N 50 mg  
         
         
Product-N°: Description labeled
(> 98%)
Quantity  
125303601 rA N 15N 10 mg  
125303602 rC N 15N 10 mg  
125303603 rG N 15N 10 mg  
125303604 rU N 15N 10 mg  
         
125303801 rA N 15N 50 mg  
125303802 rC N 15N 50 mg  
125303803 rG N 15N 50 mg  
125303804 rU N 15N 50 mg  
         
125603601 rA CN 13C, 15N 10 mg  
125603602 rC CN 13C, 15N 10 mg  
125603603 rG CN 13C, 15N 10 mg  
125603604 rU CN 13C, 15N 10 mg  
         
125603801 rA CN 13C, 15N 50 mg  
125603802 rC CN 13C, 15N 50 mg  
125603803 rG CN 13C, 15N 50 mg  
125603804 rU CN 13C, 15N 50 mg  
         
The following rNTPs will be available soon:
         
121203601 rATP C 13C 10 mg  
121203602 rCTP C 13C 10 mg  
121203603 rGTP C 13C 10 mg  
121203604 rUTP C 13C 10 mg  
121206100 rNTPs - Set C (GAUC) 13C 4 x 10 mg  
         
121203801 rATP C 13C 50 mg  
121203802 rCTP C 13C 50 mg  
121203803 rGTP C 13C 50 mg  
121203804 rUTP C 13C 50 mg  
121206200 rNTPs - Set C (GAUC) 13C 4 x 50 mg  
         

 

Silantes GmbH I Gollierstr. 70 c I 80339 München I Germany