Structural studies on large DNA and RNA molecules by nuclear magnetic resonance (NMR) require labeling of these molecules with stable isotopes. Silantes offers a wide range of stable isotope labeled ribo- and deoxyribo-nucleosidetriphosphates (rNTP, dNTP) for the synthesis of oligonucleotides.
Silantes Technology
Silantes dNTPs and rNTPs are made from DNA and RNA purified from bacteria.
The bacterial strain used is a chemolithoautotrophic organism which grows
on isotopically labeled H2, O2
and CO2. The extracted RNA or DNA is
enzymatically hydrolysed. The 5'-monophosphates are enzymatically phosphorylated
into 5'-dNTPs and 5'-rNTP and purified by ion and reversed phase HPLC.
Added Value
Our new technology for in vivo enrichment of stable isotopes enables us
to offer the ribo- and deoxyribo-nucleosidetriphosphates at very competitive
prices.
Isotope Enrichment
Silantes NTPs can be supplied in any combination of 2H, 13C and 15N.
The isotopic purity for all isotopes is >98%.
Quality
The quality of dNTPs and rNTPs is tested with respect to chemical purity
(>95%) (Fig. 1) and biological competence analysed by PCR. NTPs are
available in solution (C = 50 mM in 5 mM Tris, pH 8.0).
Conclusion
Silantes offers a full range of stable isotope labeled ribo- and deoxyribo-nucleoside-triphosphates:
· with full biological competence
· of >98% isotopic purity
· of >95% chemical purity
· at very competitive prices
"We are very happy with the 13C, 15N-labeled
RNA nucleotides that we have obtained from Silantes. We consistently receive
samples of high purity that afford high yields when used in in vitro transcription
reactions in our lab. It is also significant that we have been able to
receive relatively large amounts of labeled ribo-NTPs with minimal delays
from the time that orders are placed to the time the samples arrive in
our lab (typically less than 2-3 weeks)."
Michael F. Summers, Howard Hughes Medical
Institute, Chevy Chase, USA
"The 13C, 15N labeled
NTPs provided by Silantes were of high quality and worked very well in
our hands. I can strongly recommend to use the products by Silantes."
Prof. Harald Schwalbe, Massachusetts Institute of Technology,
Department of Chemistry, Cambridge, MA, USA
"We are really pleased by the quality of the SILANTES 13C,
15N-labeled rNTPs. At a very resonable price, they reproducibely
give yields comparable to optimized in vitro transcription reactions using
commercially available unlabeled rNTPs. No further purification needed!
And equally important, SILANTES typically delivered with minimal delays
even when we ordered larger quantities."
Mirko Hennig & James R. Williamson, The Scripps Research
Institute, La Jolla, CA
| Product-N°: | Description | labeled (> 98%) |
Quantity | |
| 201001 | dATP D | 2H | 10 mg | |
| 201002 | dCTP D | 2H | 10 mg | |
| 201003 | dGTP D | 2H | 10 mg | |
| 201004 | dTTP D | 2H | 10 mg | |
| 201005 | dNTP - Set D (GATC) | 2H | 4 x 10 mg | |
| 201101 | dATP D | 2H | 50 mg | |
| 201102 | dCTP D | 2H | 50 mg | |
| 201103 | dGTP D | 2H | 50 mg | |
| 201104 | dTTP D | 2H | 50 mg | |
| 201105 | dNTP - Set D (GATC) | 2H | 4 x 50 mg | |
| 203001 | dATP N | 15N | 10 mg | |
| 203002 | dCTP N | 15N | 10 mg | |
| 203003 | dGTP N | 15N | 10 mg | |
| 203004 | dTTP N | 15N | 10 mg | |
| 203005 | dNTP - Set N (GATC) | 15N | 4 x 10 mg | |
| 203101 | dATP N | 15N | 50 mg | |
| 203102 | dCTP N | 15N | 50 mg | |
| 203103 | dGTP N | 15N | 50 mg | |
| 203104 | dTTP N | 15N | 50 mg | |
| 203105 | dNTP - Set N (GATC) | 15N | 4 x 50 mg | |
| 206001 | dATP CN | 13C, 15N | 10 mg | |
| 206002 | dCTP CN | 13C, 15N | 10 mg | |
| 206003 | dGTP CN | 13C, 15N | 10 mg | |
| 206004 | dTTP CN | 13C, 15N | 10 mg | |
| 206005 | dNTP - Set CN (GATC) | 13C, 15N | 4 x 10 mg | |
| 206101 | dATP CN | 13C, 15N | 50 mg | |
| 206102 | dCTP CN | 13C, 15N | 50 mg | |
| 206103 | dGTP CN | 13C, 15N | 50 mg | |
| 206104 | dTTP CN | 13C, 15N | 50 mg | |
| 206105 | dNTP - Set CN (GATC) | 13C, 15N | 4 x 50 mg | |
| Product-N°: | Description | labeled (> 98%) |
Quantity | |
| 211001 | rATP D | 2H | 10 mg | |
| 211002 | rCTP D | 2H | 10 mg | |
| 211003 | rGTP D | 2H | 10 mg | |
| 211004 | rUTP D | 2H | 10 mg | |
| 211005 | rNTP - Set D (GAUC) | 2H | 4 x 10 mg | |
| 211101 | rATP D | 2H | 50 mg | |
| 211102 | rCTP D | 2H | 50 mg | |
| 211103 | rGTP D | 2H | 50 mg | |
| 211104 | rUTP D | 2H | 50 mg | |
| 211105 | rNTP - Set D (GAUC) | 2H | 4 x 50 mg | |
| 213001 | rATP N | 15N | 10 mg | |
| 213002 | rCTP N | 15N | 10 mg | |
| 213003 | rGTP N | 15N | 10 mg | |
| 213004 | rUTP N | 15N | 10 mg | |
| 213005 | rNTPs - Set N (GAUC) | 15N | 4 x 10 mg | |
| 213101 | rATP N | 15N | 50 mg | |
| 213102 | rCTP N | 15N | 50 mg | |
| 213103 | rGTP N | 15N | 50 mg | |
| 213104 | rUTP N | 15N | 50 mg | |
| 213105 | rNTPs - Set N (GAUC) | 15N | 4 x 50 mg | |
| 216001 | rATP CN | 13C, 15N | 10 mg | |
| 216002 | rCTP CN | 13C, 15N | 10 mg | |
| 216003 | rGTP CN | 13C, 15N | 10 mg | |
| 216004 | rUTP CN | 13C, 15N | 10 mg | |
| 216005 | rNTPs - Set CN (GAUC) | 13C, 15N | 4 x 10 mg | |
| 216101 | rATP CN | 13C, 15N | 50 mg | |
| 216102 | rCTP CN | 13C, 15N | 50 mg | |
| 216103 | rGTP CN | 13C, 15N | 50 mg | |
| 216104 | rUTP CN | 13C, 15N | 50 mg | |
| 216105 | rNTPs - Set CN (GAUC) | 13C, 15N | 4 x 50 mg | |