Silantes Amino Acid Mixtures - in vitro expression of proteins

Amino Acid Mixture

Silantes offers amino acid mixtures for in-vitro synthesis systems and as supplements for cell cultivation. Silantes amino acid mixtures can be supplied in any combination of ²H, ¹³C and ¹⁵N with an isotopic purity for all isotopes of >98%. The amino acids are derived by acid and enzymatic hydrolysis of labeled proteins. Since they are based on biological raw material (proteins), amino acid concentrations of different production runs may vary by 20 % . Silantes amino acid mix can be used as basis and single amino acids may be added as desired.

Amino Acid Distribution

Analysis of the amino acid distribution within Silantes amino acid mix:

Amino Acid	c(AA) [mmol/mg]	% of Total
Ala	0.5	9.2
Arg	0.3	5.5
Asn	0.3	5.5
Asp	0.5	9.2
Cys	0.1	1.8
Gln	0.3	5.5
Glu	0.5	9.2
Gly	0.4	7.3
His	0.1	1.8
Ile	0.2	3.7
Leu	0.4	7.3
Lys	0.2	3.7
Met	0.05	0.9
Phe	0.2	3.7
Pro	0.3	5.5
Ser	0.2	3.7
Thr	0.3	5.5
Trp	0.1	1.8
Tyr	0.1	1.8
Val	0.4	7.3
	5.45	100

	Description	labeled (> 98%)	Quantity
303100	In-vitro Mix (20aa) N	¹⁵N	1 g
306100	In-vitro Mix (20aa) CN	¹³C, ¹⁵N	1 g